ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (12): 2379-2389.doi: 10.11843/j.issn.0366-6964.2016.12.007

Previous Articles     Next Articles

The FAM134B, PPARγ, HSL and FAS Gene Expression Patterns and Their Association with Intramuscular Fat Content in Sheep

LUAN Zhao-jin1，2, LIU Kai-dong3, HE Jian-ning1, CHENG Ming3, QU Xu-xian4, LIU Nan1*   

  1. (1.College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China; 2. Baotou Agricultural Research Institute, Baotou 014013, China; 3. Institute of Animal Husbandry and Veterinary Medicine of Qingdao, Qingdao 266121, China; 4. Station of the Shandong Animal Husbandry and Veterinary, Jinan 250000, China)
  • Received:2016-05-18 Online:2016-12-23 Published:2016-12-23

PDF (PC)

381

Abstract:

The objectives of this study were to investigate the developmental changes of FAM134B, PPARγ, FAS and HSL gene expression in the process of sheep muscles development and their association with intramuscular fat (IMF) accumulation, to lay a theoretical foundation for studying molecular regulation mechanism of the IMF deposition in sheep. Five Aohan fine wool sheep were slaughtered at 2, 4, 5, 6 and 12 months old to collect samples from longissimus dorsi and biceps femoris, for the purpose of determining the IMF content. Then mRNA expression level of the FAM134B, PPARγ, FAS and HSL gene in different development period of muscles were analyzed by real-time PCR, and association between genes expression and IMF contents were analyzed. The results showed that: (1) Among 2-5 months old, with growing of the age, the IMF contents of longissimus dorsi and biceps femoris were increasing. Among 5-12 months old, the IMF contents remained unchanged; The IMF content of longissimus dorsi were extremely significantly higher than biceps femoris (P<0.01) at the same age. (2) In longissimus dorsi, FAM134B expression level presented first increased and then declined with growing, the highest was in 6 months old(P<0.01). In biceps femoris,FAM134B expression level presented increased and then stabilized with growing. FAS expression level increased with growing in both of two parts of muscles. PPARγ expression level presented first declined and then increased with growing. HSL expression levels was showed “declined-increased-declined” patterns in two parts of muscles. In the same month, target genes expression level had tissue-dependent in two parts of muscles.(3) Correlation analysis showed that the expression level of FAM134B was significantly positively related to FAS in two parts of muscles(P<0.05), and was negatively related to PPARγ and HSL expression levels at different levels. In longissimus dorsi, FAM134B, FAS expression levels were significantly positively related to IMF content(P<0.01 or P<0.05, respectively), PPARγ expression level was significantly negatively related to IMF content(P<0.05). In biceps femoris, FAM134B expression level was significantly positively correlated with the IMF content(P<0.05). In conclusion, FAM134B, FAS expression might have positively effect on IMF accumulation, and PPARγ, HSL expression might have negatively effect on IMF accumulation. FAM134B was likely to regulate IMF content through stimulating expression level of fat synthetic genes and inhibiting adipose decompose genes. The results would provide reference for studying related gene functions and molecular mechanism of the IMF deposition.

CLC Number: